species-specific isotype control antibody  (Biogenex)

Journal: Frontiers in Oncology

Article Title: Eosinophils Decrease Pulmonary Metastatic Mammary Tumor Growth

doi: 10.3389/fonc.2022.841921

Figure Lengend Snippet: Genetic or immunological eosinophil depletion accelerates pulmonary EO771 tumor growth. (A) Representative images of lungs harvested from WT, IL5Tg, ΔdblGATA, and Δdbl-IL5Tg mice injected i.v. with EO771 cells. Lungs were harvested 14 days post-injection due to accelerated growth in eosinophil-deficient ΔdblGATA and Δdbl-IL5Tg mice. (B) WT, IL5Tg, ΔdblGATA, and Δdbl-IL5Tg left lung lobe weights and EO771 tumor area quantification as a proportion of total lung area of right lung lobes. Data is from one independent experiment, which has been replicated using EO771-GFP cells. (C) Frequency of IFNγ + and TNFα + ex vivo stimulated CD8 + lung T cells harvested from WT, IL5Tg, ΔdblGATA, and Δdbl-IL5Tg mice injected i.v. with EO771 cells. (D) Representative images of lungs from WT mice injected i.v. with EO771 cells and treated the next day with 0.6mg/kg α-Siglec-F or IgG 2a isotype control and every 5 days thereafter. Lungs were harvested 13 days post-i.v. EO771 injection. (E) Quantification of EO771 tumor area as a proportion of total lung area of right lung lobes. Data points are individual mice with mean ± SEM of 4-10 mice per group. *p < 0.05; **p < 0.01; ****p < 0.0001.

Article Snippet: To deplete eosinophils, mice were treated via intraperitoneal injection with 0.6mg/kg anti-Siglec-F (238047, R&D Systems) or IgG 2A isotype control (54447, R&D Systems).

Techniques: Injection, Ex Vivo, Control

Journal: Aging Cell

Article Title: The aged hematopoietic system promotes hippocampal‐dependent cognitive decline

doi: 10.1111/acel.13192

Figure Lengend Snippet: Cyclophilin A regulates cellular and cognitive hallmarks of hippocampal aging. (a) Schematic showing hydrodynamic tail vein injection (HDTVI)‐mediated CyPA overexpression (OE) paradigm in young mice. (b–d) Young CyPA‐OE or GFP control mice were tested in novel object recognition (b; NOR; n = 8–12/group), contextual fear conditioning (c; FC; n = 15–16), and cued FC (d; n = 15–16). (e, f) Representative fields (e; scale bar = 100 μm) and quantification (f) of DCX+ cells ( n = 11/group), in DG of young CyPA‐OE and GFP control mice. (g, h) Representative Western blot (g) and quantification (h) of hippocampal lysates from young CyPA‐OE and GFP control mice, probed with anti‐NR2B, synapsin‐1 (Syn‐1), synaptophysin (Syp), and GAPDH ( n = 5/group). (i) Schematic showing CyPA inhibition paradigm in aged mice. (j–l) Aged mice treated with an anti‐CyPA antibody or IgG isotype control (ctrl) were tested in NOR (j; n = 10–14/group), contextual FC (k; n = 10–14/group), and cued FC (l; n = 10–14/group). (m, n) Representative fields (m; scale bar = 100 μm) and quantification (n) of DCX+ ( n = 8–10/group), in anti‐CyPA or IgG ctrl‐treated old mice. (o, p) Representative Western blot (o) and quantification (p) of hippocampal lysates from anti‐CyPA or IgG ctrl‐treated old mice, probed with anti NR2A, Syn‐1, Syp, and GAPDH ( n = 5/group). All data shown as the mean + SEM . *p < 0.05. t test (c, d, f, h, k, l, n, p). One‐sample t test vs. hypothetical mean of 50 (b, j).

Article Snippet: The anti‐CyPA antibody, 8H7 (Von Ungern‐Sternberg et al., ), or a monoclonal IgG2A isotype control (R&D Systems) was intraperitoneally administered to aged (19 months) mice (20 μg/kg).

Techniques: Injection, Over Expression, Control, Western Blot, Inhibition